iBind Automated Western Detection Systems
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1. iBind Western Device
The iBind Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays.
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The iBind Western Device works exclusively with iBind Cards and the iBind Solution Kit or iBind Fluorescent Detection (FD) Solution Kit to process one mini-membrane for immunodetection. The iBind device provides automated convenience and superior reproducibility over traditional manual methods. Each solution is released from the iBind device wells to an iBind Card via SLF in ~2.5 hours. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions over the membrane, increasing the antigen-antibody interaction.
Detection Method: | Chemiluminescent, |
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Membrane Compatibility: | PVDF and Nitrocellulose |
Run Time: | ~2.5 hours |
Capacity: | 1 mini blot (8 x 8 cm) |
Product Line: | iBind |
Product Size: | 1 device |
2. iBind Flex Western Device
The iBind Flex Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays. Each solution is released from the iBind Flex device wells to an iBind Flex Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.
The iBind Flex western detection procedure minimizes hands-on time and is highly reproducible. Setup time is fast: within 2.5 h you are ready for the detection step. Compared to manual methods, the iBind Flex western system requires less primary antibody, enables reproducible results, and is compatible with downstream detection protocols.
The iBind Flex Western Device includes three user-interchangeable well inserts to process up to one midi blot, two mini blots, or six vertically-cut strip blots when used with the iBind Flex Cards and iBind Flex Solution Kit or iBind Flex Fluorescent Detection (FD) Solution Kit.
Detection Method: | Chemiluminescent, |
---|---|
Membrane Compatibility: | PVDF and Nitrocellulose |
Run Time: | ~2.5 hours |
Capacity: | one midi blot, six vertically-cut strip blots, two mini blots |
Product Line: | iBind Flex |
Product Size: | 1 device |
Shipping Condition: | Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.
3. iBind Western Starter Kit
The iBind Western Starter Kit includes an iBind Western Device as well as all of the consumables and reagents necessary to get started using this automated western-processing device. The iBind Western Device performs every step in the western protocol from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays.
The iBind Western Device works exclusively with iBind Cards and the iBind Solution Kit to process one mini-membrane for immunodetection. The iBind device provides automated convenience and superior reproducibility over traditional manual methods. Each solution is released from the iBind device wells to an iBind Card via SLF in ~2.5 hours. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions over the membrane, increasing the antigen-antibody interaction.
제품구성
• iBind™ Western Device 1개
• iBind™ Card 10개(실온 보관)
• iBind™ Solution Kit 1개(보관 온도: 2–8°C)
4. iBind Flex Western Starter Kit
The iBind Flex Western Starter Kit for fluorescent detection includes an iBind Flex Western Device , iBind Flex Cards, and an iBind Flex Fluorescent Detection (FD) Solution Kit . The iBind Flex Western Device is a small benchtop device that automates western blot processing (immunodetection). It applies mechanical pressure to an iBind Flex Card to generate sequential lateral flow (SLF) of immunodetection solutions for the blocking, antibody binding, and washing steps. The glass fiber matrix of the iBind Flex Card allows for homogenous and consistent flow of the solutions over the membrane, increasing the antigen-antibody interaction.
The iBind Flex Western Device allows all solutions to be prepared and loaded in the device at the start of the procedure; all subsequent steps proceed automatically through SLF action—no electricity or batteries required. Blots are ready for the final detection steps in approximately 2.5 hours.
The iBind Flex Western Device includes three user-interchangeable well inserts to process up to one midi blot, two mini blots, or six vertically-cut strip blots when used with iBind Flex Cards, and the iBind Flex Solution Kit or iBind Flex Fluorescent Detection (FD) Solution Kit to process one mini-blot.
제품구성
• 1 iBind Flex Western Device
• 10 iBind Flex Cards, store at room temperature
• 1 iBind Flex Fluorescent Detection (FD) Solution Kit, store at 2–8°C
Thermo Fisher Scientific Invitrogen "How to automate your western blot processing using the Invitrogen iBind Western Systems" Youtube 동영상 바로가기 (클릭)
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Western-Blotting-Workflow_진트론바이오텍.pdf (6.3M)
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