Absolutely attainable chip-based digital PCR 

QuantStudio 3D Digital PCR System

The Applied Biosystems™ QuantStudio™ 3D Digital PCR System leverages high-density nanofluidic chip technology for detecting rare events, analyzing small differences between two targets, or counting the exact number of targets in a sample. The higher precision, sensitivity, and absolute nature of digital PCR is ideal for research in:

• Qualification of molecular standards used in traditional real-time PCR experiments

• Pathogen detection and load determination

• Rare-target detection such as somatic mutation detection in oncology research

• GMO detection and contamination assessment

• Generation of references and standards

• Copy number variation

Simple workflow

A streamlined workflow with minimal sample handling enables turnkey processing. Just load and go.

Affordable

Less than half the price of competing platforms, making digital PCR technology within reach for most labs.

Absolute quantification

20,000 reaction wells yield data in copies/μL, enabling high precision and sensitivity without the need for a standard curve.

Sealed system

Helps limit contaminants with a sealed chip and no exposed sample transfer steps.

Easily fits in populated spaces

Small footprint (7 x 5 x 9 in.) will fit most benchtops and can be moved when needed.

Compatible

Use your existing Applied Biosystems™ TaqMan® Real-Time PCR Assays for a digital result.

Application spotlight

Determining low copy number in equivocal tissue

Many cancers are regulated by mutations in a specific gene or group of genes, or copy number changes. These aberrations may be associated with aggressiveness of the disease or prognosis. Research using digital PCR provides a fast and easy workflow to precisely identify low copy numbers that have small differences. Compared to immunohistochemistry by fluorescence in situ hybridization, digital PCR is less impacted by tissue heterogeneity and provides clearer research results.