StepOnePlus Real-Time PCR System
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Remarkably Simple Systems. Simply Remarkable Results.
StepOne™ and StepOnePlus™ Real-Time PCR Systems
Remarkably Simple Systems
Applied Biosystems, a leader in technology solutions for life science research, introduces its latest innovations in real-time PCR—the StepOne™ (48-well) and StepOnePlus™ (96-well) Real-Time PCR Systems. These remarkably simple real-time PCR systems are specifically designed with a user-friendly yet powerful interface for both new and experienced real-time PCR researchers.
System Features
The StepOne™ and StepOnePlus™ Systems bring advanced real-time PCR technology to a new level of accessibility—even for first-time users. Both systems measure amplification as it occurs, cycle by cycle, allowing for precise and quantitative measurements during the exponential phase of PCR. Beginning at the homepage (Figure 1), you can navigate seamlessly through all aspects of the real-time PCR process including sample and reaction setup, thermal cycling, and fluorescent detection. Focused application software analyzes and interprets experimental results. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the software (optional). The system provides 2-fold discrimination with 99.7% confidence, along with 9 logs of dynamic range. Highly sensitive, these systems detect 10 copies of RNase P in a 30-μL reaction. Additionally, for laboratories with the StepOne™ System that require greater throughput, an upgrade kit is available.*
StepOnePlus™ Real-Time PCR System Upgrade Kit
To accommodate your changing real-time PCR needs, Applied Biosystems offers the StepOnePlus™ Real-Time PCR System Upgrade Kit. The upgrade converts a StepOne™ System into a StepOnePlus™ System by upgrading the 48-well block to a 96-well VeriFlex™ Block and expanding the dye flexibility to four colors. The color of the bar above the block is also updated to indicate that the instrument is now a StepOnePlus™ System. Just send in your existing StepOne™ System and you’ll receive a StepOnePlus™ loaner instrument to use until your upgraded system arrives in your lab.
Instrument Chemistries
Both StepOne™ Systems are equipped with fluorophore detection chemistries that include FAM™ and VIC® dye-labeled TaqMan® MGB probe-based assays, VIC® and TAMRA™ dye-labeled probe-based assays, and SYBR® Green I dye chemistry. [Note: the TAMRA™ dye is available only with the StepOnePlus™ System.] TaqMan® probe-based assays provide outstanding specificity and sensitivity, and SYBR® Green I dye chemistry is an economical alternative for target identification, initial screening assays, or assays that require only a few reactions.
Applications
The StepOne™ Systems support any real-time PCR application. Predesigned or custom assays exist for the following applications:
• SNP genotyping • Translocation analysis
• Gene expression profiling • Gene detection
• MicroRNA expression • Viral load analysis
• Methylation • Mutation scanning
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26회 다운로드 | DATE : 2020-12-10 10:06:56
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